Analysis Note

ControlHeLa cell lysate

Application

Detect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-4 in human breast carcinoma and skin tissues.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.

Immunogen

Linear peptide corresponding to human CHD-4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-4 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~220 kDa observed